DNA

Part:BBa_K4399014:Design

Designed by: Duoqing LIN   Group: iGEM22_Worldshaper-Shanghai   (2022-10-04)


XVE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 798
    Illegal PstI site found at 982
    Illegal PstI site found at 1153
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 982
    Illegal PstI site found at 1153
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 932
    Illegal BamHI site found at 501
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 798
    Illegal PstI site found at 982
    Illegal PstI site found at 1153
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 798
    Illegal PstI site found at 982
    Illegal PstI site found at 1153
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

XVE is a constitutively expressed transcription factor that could be activated in presence of β-estrogen.

Source

The sequence is based on BBa_P10300。

References